Effects of in ovo injection of nanocurcumin and vitamin E on antioxidant status, immune responses, intestinal morphology and growth performance of broiler chickens exposed to heat stress

Document Type : Original Research Article (Regular Paper)

Authors

1 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

The aim of this study was to investigate the effects of in ovo injection of nanocurcumin (NC) and vitamin E (VE) on antioxidant status, intestinal histomorphology and growth performance of the hatched chicks. Fertile eggs (n=352) were allocated to 8 treatments with 4 replicates of 11 eggs each. At d 17.5 of incubation, the eggs were injected with 0.01, 0.03 and 0.05 mL/egg of NC and 0.03, 0.06 and 0.09 mL/egg VE solution into the amniotic cavity, and the rest two groups were used as sham and un-injected controls. The hatchlings from each treatment were randomly assigned to 4 replicates of 10 birds and reared until d 24 of age under heat stress (8 h daily at 35oC). The experimental design used was a completely randomized one. Results showed that superoxide dismutase activity in liver was increased (P<0.01) by IOI of 0.05 mL/egg NC in compare with control and sham groups on d 10. Total antioxidant capacity was decreased (P<0.05) by IOI of 0.03 and 0.06 mL/egg VE in compare with the control group. Liver glutathione peroxidase and malondialdehyde activities, blood heterophil (H) and lymphocyte (L) counts and H/L ratio, serum total cholesterol, HDL, LDL and VLDL levels and also crypt depth (CD) were not significantly affected by treatments on d 10. Villous height (VH) in the birds hatched from 0.01 and 0.03 mL/egg NC treatments were shorter than control group. The VH to CD ratio in NC treatments and 0.03 and 0.06 mL/egg VE were lower than controls (P<0.01). In ovo administration of NC and VE had no significant effects on growth performance of broiler chickens during 1-10 days of age; but decreased (P<0.05) feed intake during 1-24 d without affecting body weight gain and feed conversion ratio. Hatchability in 0.03 and 0.05 mL/egg NC treatments were decreased (P<0.05) in compare with the control treatment. In general, in ovo administration of NC improved antioxidant status of the hatchlings. In ovo injection of NC and VE didn’t have a significant effect on posthatch growth performance of the broiler chickens.

Keywords

Main Subjects

References

  • Anand, P., Kunnumakkara, A.B., Newman, R.A., Aggarwal, B.B., 2007. Bioavailability of curcumin: problems and promises. Molecular Pharmaceutics 4, 807-818.
  • Aviagen, 2014. Nutrition Specification for Ross 308. Aviagen Limited, Newbridge, Scotland, UK.
  • Aygun, A., 2016. The effects of in ovo injection of propolis on egg hatchability and starter live performance of Japanese quails. Brazilian Journal of Poultry Science 18, 83-89.
  • Basavaraj, M., Nagabhushana, V., Prakash, N., Appannavar, M.M., Wagmare, P., Mallikarjunppa, S., 2011. Effect of dietary supplementation of Curcuma longa on the biochemical profile and meat characteristics of broiler rabbits under summer stress. Veterinary World 4, 15-18.
  • Bisht, S., Feldmann, G., Soni, S., Ravi, R., Karikar, C. Maitra, A., Maitra, A., 2007. Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"): a novel strategy for human cancer therapy. Journal of Nanobiotechnology 5, 1-18.
  • Bolukbasi, S.C., Erhan, M.K., Keles, M.S., Kocyigit, R., 2007. Effect of dietary vitamin E on the performance, plasma and egg yolk vitamin E levels and lipid oxidation of egg in heat stressed layers. Journal of Applied Biological Sciences 1, 19-23.
  • Brenes, A., Viveros, A., Goni, I., Centeno, C., Saura Calixto, F., Arija, I., 2008. Effect of grape seed extract on growth performance protein and polyphenol digestibilities and antioxidant activity in chickens. Spanish Journal of Agricultural Research 8, 326-333.
  • Burke, W.H., 1992. Sex differences in incubation length and hatching weights of broiler chicks. Poultry Science 71, 1933-1938.
  • Durrani, F.R., Mohammed, I., Asal, S., Shhail, S.M., Naila, C., Durrani, Z., 2006. Effect of different levels of feed added turmeric (Curcuma longa) on the performance of broiler chicks. Agricultural Biology Science 1, 9-11.
  • Emadi, M., Kermanshahi, H., Maroufyan, E., 2007. Effect of varying levels of turmeric rhizome powder on some blood parameters of broiler chickens fed corn soybean meal based diets. International Journal of Poultry Science 6, 345-348.
  • Ghazanfarpoor, R., Talebi, A., 2013. Protective role of heat shock proteins in cells under stress conditions. Science International Journal 1, 350-355.
  • Giannenas, D.T., Tsalie, E., Chronis, E.F., Doukas, D., Kyriazakis, I., 2010. Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens. Research in Veterinary Science 89, 78-84.
  • Habibi, R., Sadeghi, G., Karimi, A., 2014. Effect of different concentrations of ginger root powder and its essential oil on growth performance, serum metabolites and antioxidant status in broiler chicks under heat stress. British Poultry Science 55, 228-237.
  •  Hajati, H., Hassanabadi, A., Golian, A., Nassiri Moghaddam, H., Nassiri, M.R., 2014. The effect of in ovo injection of grape seed extract and vitamin C on hatchability, antioxidant activity, yolk sac weight, performance and ileal microflora of broiler chickens. Research Opinions in Animal and Veterinary Sciences4, 633-638.
  • Hajati, H., Hassanabadi, A., Golian, A., Nassiri Moghaddam, H., Nassiri, M.R., 2018. The effect of grape seed extract supplementation on performance, antioxidant enzyme activity, and immune responses in broiler chickens exposed to chronic heat stress. Iranian Journal of Applied Animal Science 8, 109-117.
  • Heckert, R.A., Estevez, I., Russekcohen, E., Pettitriley, R., 2002. Effects of density and perch availability on the immune status of broilers. Poultry Science 81, 451-457.
  • Hosseini-Vashan, S.J., Golian, A., Yaghobfar, A. 2015. Effects of turmeric rhizome powder and source of oil in diet on blood metabolites, immune system and antioxidant status in heat stressed broiler chickens. Journal of Livestock Science and Technologies 3, 13-20.
  • Hosseini-Vashan, S.J., Golian, A., Yaghobfar, A., Zarban, A., Afzali, N., Esmaeilinasab, P., 2012. Antioxidant status, immune system, blood metabolites and carcass characteristic of broiler chickens fed turmeric rhizome powder under heat stress. African Journal of Biotechnology 11, 16118-16125.
  • Itallo, C.S.A., Marcos, B.C., Raiana, A.N., Julyana, M.S.M., Cirano, J.U., Gabriela, C.G., Matheus, M.R., Nadja, S.M.L., 2019. Effect of vitamin E in ovo feeding to broiler embryos on hatchability, chick quality, oxidative state, and performance. Poultry Science 98, 3652-3661.
  • Kadam, M., Barekatain, M.M.R., K-Bhanja, S., Iji, P.A., 2013. Prospects of in ovo feeding and nutrient supplementation for poultry: The science and commercial applications-a review. Journal of the Science of Food and Agriculture 93, 3654-3661.
  • Khaligh, F., Hassanabadi, A., Nassiri Moghaddam, H., Golian, A., Kalidari, G.A., 2017. Effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic oxidative status and early post-hatch performance of broiler chicks. Journal of Animal Physiology and Animal Nutrition 102, 413-420.
  • Kim, M., Kim, Y., 2010. Hypocholesterolemic effects of curcumin via up-regulation of cholesterol 7a-hydroxylasein rats fed a high fat diet. Nutrition Research Practice 4, 191-195.
  • Malheiros, R.D., Ferket, P.R., Goncalves, F.M., 2012. Oxidative stress protection of embryos by “In ovo” supplementation. Proceedings of the 24thWorld’s Poultry Congress, 5-9 August, Salvador-Bahia, Brazil.
  • Mehala, C., Moorthy, M., 2008. Effect of Aloe vera and Curcuma longa (turmeric) on carcass characteristics and biochemical parameters of broilers. International Journal of Poultry Science 7, 857-861.
  • Menon, V.P., Sudheer, A.R., 2007. Antioxidant and anti-inflammatory properties of curcumin. Advances in Experimental Medicine and Biology 595, 105-125.
  • Nowaczewski, S., Kontecka, H., Krystianiak, S., 2012. Effect of in ovo injection of vitamin C during incubation on hatchability of chickens and ducks. Folia Biology (Krakow) 60, 93-97.
  • Oso, A.O., Suganthi, R.U., Reddy, G.B.M., Malik, P.K., Thirumalaisamy, G., Awachat, V.B., Selvaraju, S, Arangasamy, A., Bhatta, R., 2019. Effect of dietary supplementation with phytogenic blend on growth performance, apparent ileal digestibility of nutrients, intestinal morphology, and cecal microflora of broiler chickens. Poultry Science 98, 4755-4766.
  • Rahmani, M., Golian, A., Kermanshahi, H., Bassami, M.R., 2017. Effects of curcumin and nanocurcumin on growth performance, blood gas indices and ascites mortalities of broiler chickens reared under normal and cold stress conditions. Italian Journal of Animal Science 16, 438-446.
  • Rajput, N., Muhammad, N., Yan, R., Zhong, X., Wang, T., 2013. Effect of dietary supplementation of curcumin on growth performance, intestinal morphology and nutrients utilization of broiler chicks. Journal of Poultry Science 50, 44-52.
  • Rajsekhar, P.B., Bharani, R.S., Angel, K., Ramachandran, M., Rajsekhar, S.P.V., 2015. Curcumin Nanoparticles: A Therapeutic Review. Research Journal of Pharmaceutical, Biological and Chemical Sciences 6, 1180-1185.
  • Ricks, C.A., Avakian, A., Bryan, T., Gildersleeve, R., Haddad, E., Ilich, R., King, S., Murray, L., Phelps, P., Poston, R., Whitfill, C., Williams, C., 1999. In ovo vaccination technology. Advances in Veterinary Medicine 41, 495-515.
  • Rukkumani, R., Aruna, K., Varma, P.S., Rajasekaran, K.N., Menon, V.P., 2004. Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress. Journal of Pharmacological Sciences 7, 274-283.
  •  Salary, J., Sahebi-Ala, F., Kalantar, M., Hemati Matin, H.R. 2014. In ovo injection of vitamin E on post-hatch immunological parameters and broiler chicken performance. Asian Pacific Journal of Tropical Biomedicine 4, 616-619.
  • SAS, 2012. SAS User’s Guide: Statistics. Version 9.4. SAS Institute Inc., Cary, North Carolina. USA.
  • Satoh, K., 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 90, 37-43.
  • Sirovina, D., Orsolic, N., Koncic, M.Z., Kovacevic, G., Benkovic, V., Gregorovic, G., 2013. Quercetin vs Chrysin: Effect on liver histopathology in diabetic mice. Human and Experimental Toxicology 32, 1058-1066.
  • Surai, P.F., Fisinin, V.L., 2012. Feeding breeders to avoid oxidative stress in embryos. Proceedings of the 24thWorld’s Poultry Congress, 5-9 August, Salvador-Bahia, Brazil.
  • Suvanated, C., Kijparkorn, S., Angkanaporn, K., 2003. Effect of turmeric (Curcuma longa linn.) as an antioxidant on immune status and growth performances of stressed broilers. PhD Thesis, Bangkok, Thailand: Faculty of Veterinary Science, Chulalongkorn University.
  • Tayer, M., Nobakht, A., Pishjangh, J., 2012. Effects of different levels of green grape (Vitis vinifera) leaves on performance, carcass traits, blood biochemical and immunity parameters of broilers. Australian Journal of Basic Applied Science 3, 103-111.
  • Triplett, M.D.,  Zhai,  W.,  Peebles, E.D.,  McDaniel, C.D., Kiess, A.S., 2018. Investigating commercial in ovo technology as a strategy for introducing probiotic bacteria to broiler embryos. Poultry Science 97, 658-666.
  • Uni, Z., Yadgary, L., Yair, R., 2012. Nutritional limitations during poultry embryonic development. Journal of Applied Poultry Research 21, 175-184.
  • Urso, U.R., Dahlke, F., Maiorka, A., Bueno, I.J., Schneider, A.F., Surek, D., Rocha, C., 2015. Vitamin E and selenium in broiler breeder diets: Effect on live performance, hatching process, and chick quality. Poultry Science 94, 976-983.
  • Wang, D., Huang, H., Zhou, L., Li, W., Zhou, H., Hou, G., Liu, J., Hu, L., 2015. Effects of dietary supplementation with turmeric rhizome extract on growth performance, carcass characteristics, antioxidant capability, and meat quality of Wenchang broiler chickens. Italian Journal of Animal Science 14, 344-349.
  • Zakaria, A.H., Allatif, A.A., Alanezi, M.A., 1998. Effect of ascorbic acid on embryonic development, hatch time and growth of extended delayed placement of broiler chickens. European Poultry Science 62, 11-15.
  • Zhang, H., Elliott, K.E.C., Durojaye, O.A., Fatemi, S.A., Peebles, E.D., 2018. Effects of in ovo administration of L-ascorbic acid on broiler hatchability and its influence on the effects of pre-placement holding time on broiler quality characteristics. Poultry Science 97, 1941-1947.
Journal of Livestock Science and Technologies
Volume 8, Issue 1
June 2020
Pages 17-27

Files

Share

How to cite

Statistics