TY - JOUR ID - 2636 TI - Effects of in ovo injection of nanocurcumin and vitamin E on antioxidant status, immune responses, intestinal morphology and growth performance of broiler chickens exposed to heat stress JO - Journal of Livestock Science and Technologies JA - JLST LA - en SN - 2322-3553 AU - Heidary, Mahboob AU - Hassanabadi, Ahmad AU - Mohebalian, Hadi AD - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. AD - Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Y1 - 2020 PY - 2020 VL - 8 IS - 1 SP - 17 EP - 27 KW - broiler KW - Heat stress KW - in ovo injection KW - nanocurcumin KW - vitamin E DO - 10.22103/jlst.2020.15352.1310 N2 - The aim of this study was to investigate the effects of in ovo injection of nanocurcumin (NC) and vitamin E (VE) on antioxidant status, intestinal histomorphology and growth performance of the hatched chicks. Fertile eggs (n=352) were allocated to 8 treatments with 4 replicates of 11 eggs each. At d 17.5 of incubation, the eggs were injected with 0.01, 0.03 and 0.05 mL/egg of NC and 0.03, 0.06 and 0.09 mL/egg VE solution into the amniotic cavity, and the rest two groups were used as sham and un-injected controls. The hatchlings from each treatment were randomly assigned to 4 replicates of 10 birds and reared until d 24 of age under heat stress (8 h daily at 35oC). The experimental design used was a completely randomized one. Results showed that superoxide dismutase activity in liver was increased (P<0.01) by IOI of 0.05 mL/egg NC in compare with control and sham groups on d 10. Total antioxidant capacity was decreased (P<0.05) by IOI of 0.03 and 0.06 mL/egg VE in compare with the control group. Liver glutathione peroxidase and malondialdehyde activities, blood heterophil (H) and lymphocyte (L) counts and H/L ratio, serum total cholesterol, HDL, LDL and VLDL levels and also crypt depth (CD) were not significantly affected by treatments on d 10. Villous height (VH) in the birds hatched from 0.01 and 0.03 mL/egg NC treatments were shorter than control group. The VH to CD ratio in NC treatments and 0.03 and 0.06 mL/egg VE were lower than controls (P<0.01). In ovo administration of NC and VE had no significant effects on growth performance of broiler chickens during 1-10 days of age; but decreased (P<0.05) feed intake during 1-24 d without affecting body weight gain and feed conversion ratio. Hatchability in 0.03 and 0.05 mL/egg NC treatments were decreased (P<0.05) in compare with the control treatment. In general, in ovo administration of NC improved antioxidant status of the hatchlings. In ovo injection of NC and VE didn’t have a significant effect on posthatch growth performance of the broiler chickens. UR - https://lst.uk.ac.ir/article_2636.html L1 - https://lst.uk.ac.ir/article_2636_a1bd86d5b4fb2c7ff00d2ca866430b53.pdf ER -