Effects of unprotected and calcium salts of palm and linseed oils on feed intake, total tract digestibility, ruminal degradation and fermentation in lambs

Document Type : Original Research Article (Regular Paper)

Authors

1 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran

3 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

The objective of present study was to assess the effects of unprotected and calcium salts of oils on total tract digestibility, ruminal degradation and fermentation in lambs. Four lambs fitted with ruminal fistula were used in a change-over design using 2 × 2 factorial arrangement. Levels of first factor (oils) were palm oil and linseed oil and levels of second factor were protected oil (calcium salts) and unprotected oil. Feeding of palm oil resulted in higher ruminal effective degradability of neutral detergent fiber (NDF) than linseed oil, and lambs fed protected oil had higher ruminal effective degradability of NDF than unprotected oil (P<0.05). Lambs fed palm oil had higher dry matter intake (DMI) and total tract digestibility of nutrients compared to those fed linseed oil. Acetate concentration in lambs fed palm oil was higher compared to linseed oil. Feeding of protected oil resulted in higher acetate concentration than unprotected oil (P<0.05). The lowest acetate concentration was found in lambs fed unprotected linseed oil (ULO) and the highest acetate concentration was observed in lambs fed unprotected and protected palm oil (UPO and PPO, respectively). Lambs fed palm oil had the highest gas and methane production compared to linseed oil. Protected oil resulted in higher gas and methane production than unprotected oil (P<0.05).  Lambs fed PPO had the highest, and those fed ULO had the lowest methane production (P<0.05). Based on the results, protection of linseed oil is necessary to enhance ruminal degradation and fermentation, but this process is not necessary for palm oil. 

Keywords

Main Subjects


References
Ammah, A.A., Benchaar, C., Bissonnette, N., Gévry, N., Ibeagha-Awemu, E.M., 2018. Treatment and post-treatment effects of dietary supplementation with safflower oil and linseed oil on milk components and blood metabolites of Canadian Holstein cows. Journal of Applied Animal Research 46, 898-906 .https://doi.org/10.1080/09712119.2017.1422256.
AOAC, 2005. Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists, Washington, DC. USA.
Beauchemin, K.A., McGinn, S.M., Petit, H.V., 2007. Methane abatement strategies for cattle: Lipid supplementation of diets. Canadian Journal of Animal Science 87, 431-440.
Behan, A.A., Loh, T.C., Fakurazi, S., Kaka, U., Kaka, A., Samsudin, A.A., 2019. Effects of supplementation of rumen protected fats on rumen ecology and digestibility of nutrients in sheep. Animals (Basel) 9, 400. doi: 10.3390/ani9070400.
Benchaar, C., Hassanat, F., Martineau, R., Gervais, R., 2015. Linseed oil supplementation to dairy cows fed diets based on red clover silage or corn silage: Effects on methane production, rumen fermentation, nutrient digestibility, N balance, and milk production. Journal of Dairy Science 98, 7993-8008. doi:10.3168/jds.2015-9398.
Block, E., Kilmer, L.H., Muller, L.D., 1981. Acid insoluble ash as a marker of digestibility for sheep fed corn plants or hay and for lactating dairy cattle fed hay. Journal of Animal Science 52, 1164-1169. doi: 10.2527/jas1981.5251164x. PMID: 7195395.
 Cannas, A., Tedeschi, L.O., Fox, D.G., Pell, A.N., Van Soest, P.J., 2004. A mechanistic model for predicting the nutrient requirements and feed biological values for sheep. Journal of Animal Science 82, 149-169. doi: 10.2527/2004.821149x.
Chamberlain, M.B., DePeters, E.J., 2017. Impacts of feeding lipid supplements high in palmitic acid or stearic acid on performance of lactating dairy cows. Journal of Applied Animal Research 45, 126-135. https://doi.org/10.1080/09712119.2015.1124327.
Demirel, G., Wachira, A.M., Sinclair, L.A., Wilkinson, R.G., Wood, J.D., Enser, M., 2004. Effects of dietary n-3 polyunsaturated fatty acids, breed and dietary vitamin E on the fatty acids of lamb muscle, liver and adipose tissue. British Journal of Nutrition 91, 551–565. doi: 10.1079/BJN20031079.
Duckett, S.K., Gillis, M.H., 2010. Effects of oil source and fish oil addition on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. Journal of Animal Science 88, 2684–2691.
Fenner, H., 1965. Method for determining total volatile bases in rumen fluid by steam distillation. Journal of Dairy Science 48, 249-251.
Fiorentini, G., Carvalho, I.P., Messana, J.D., Castagnino, P.S., Berndt, A., Canesin, R.C., Frighetto, R.T., Berchielli, T.T., 2014. Effect of lipid sources with different fatty acid profiles on the intake, performance, and methane emissions of feedlot Nellore steers. Journal of Animal Science 92, 1613–1620.
Francisco, A.E., Santos-Silva, J.M., Portugal, A.P., Alves, S.P., Bessa, R.J., 2019. Relationship between rumen ciliate protozoa and biohydrogenation fatty acid profile in rumen and meat of lambs. PLoS One 14, e0221996. doi: 10.1371/journal.pone.0221996.
Harvatine, K.J., and Allen, M.S., 2006. Fat supplements affect fractional rates of ruminal fatty acid biohydrogentaion and passage in dairy cows. The Journal of Nutrition 136, 677–685.
He, Y., Niu, W., Qiu, Q., Xia, C., Shao, T., Wang, H., Li, Q., Yu, Z., Gao, Z., Rahman, M.A.U., Su, H., Cao, B. 2018. Effect of calcium salt of long-chain fatty acids and alfalfa supplementation on performance of Holstein bulls. Oncotarget 9, 3029-3042. doi: 10.18632/oncotarget.23073.
Hristov, A.N., Oh, J., Lee, C., Meinen, R., Montes, F., Ott, T., 2013. Mitigation of Greenhouse Gas Emissions in Livestock Production – A Review of Technical Options for Non-CO Emissions (FAO Animal Production and Health Paper No.177).  Rome: FAO.
 Jacob, A.B., Balakrishnan,  V., Kathirvelan,  C., 2012. Effect of amount and source of vegetable oils in a high fibrous cattle diet on in vitro rumen fermentation, nutrient degradability and rumen cis-9, trans-11 CLA concentration. Journal of Applied Animal Research 40, 148-153. doi: 10.1
Lamp, O., Reyer, H., Otten, W., Nürnberg, G., Derno, M., Wimmers, K., Metges, C.C., Kuhla, B. 2018. Intravenous lipid infusion affects dry matter intake, methane yield, and rumen bacteria structure in late-lactating Holstein cows. Journal of Dairy Science 101, 6032-6046. doi: 10.3168/jds.2017-14101.
Lourenço, M., Ramos-Morales, E., Wallace, R.J., 2010. The role of microbes in rumen lipolysis and biohydrogenation and their manipulation. Animal 4, 1008–1023. doi:10.1017/S175173111000042X
Lashkari, S., Bonefeld-Petersen, M., Krogh-Jensen, S., 2019. Rumen biohydrogenation of linoleic and linolenic acids is reduced when esterified to phospholipids or steroids. Food Science and Nutrition 8, 79-87. doi: 10.1002/fsn3.1252.
Ma, Y., Chen, X., Zahoor Khan, M., Xiao, J., Liu, S., Wang, J., Alugongo, G.M., Cao, Z. 2022. Biodegradation and hydrolysis of rice straw with corn steep liquor and urea-alkali pretreatment. Frontier Nutrition 9, 989239. doi: 10.3389/fnut.2022.989239.
Maia, M.R., Chaudhary, L.C., Bestwick, C.S., Richardson, A.J., McKain, N., Larson, T.R., Graham, I.A., Wallace, R.J. 2010. Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. BMC Microbiology 10, 52. doi: 10.1186/1471-2180-10-52.
Martin, C., Rouel, J., Jouany, J.P., Doreau, M., Chilliard, Y., 2008. Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil. Journal of Animal Science 86, 2642-2650
Messana, J. D., Berchielli, T. T., Arcuri, P. B., Ribeiro, A. F., Fiorentini, G., Canesin, R.C., 2012. Effects of different lipid levels on protozoa population, microbial protein synthesis and rumen degradability in cattle. doi: 10.4025/actascianimsci.v34i3.12729. Acta Scientiarum. Animal Sciences 34, 279-285. https://doi.org/10.4025/actascianimsci.v34i3.12729.
Nagaraja, T.G., Newbold, C.J., Van Nevel, C.J., 1997. Manipulation of ruminal fermentation. In Hobson, N.P. (Ed.), Rumen Microbial Ecosystem. Blackie Publishing, Inc., London, pp. 523-631.
Naik, P.K., Saijpaul, S., Rani, N., 2009. Effect of ruminally protected fat on in vitro fermentation and apparent nutrient digestibility in buffaloes (Bubalus bubalis). Animal Feed Science and Technology 153, 68–76, doi: 10.1016/j.anifeedsci.2009.06.008.
Nocek, J.E., 1988. In situ and other methods to estimate ruminal protein and energy digestibility: a review. Journal of Dairy Science 71, 2051-2069.
Oliveira, A.P., Cunha, C.S., Pereira, E.S., Biffani, S., Medeiros, A.N., Silva, A.M.A., Marcondes, M.I. 2020. Meta-analysis of dry matter intake and neutral detergent fiber intake of hair sheep raised in tropical areas. PLoS One 15, e0244201. doi: 10.1371/journal.pone.0244201.
Ørskov, E.R., McDonald, I., 1979. The estimation of protein degradability in the rumen fromincubation measurements weighted according to rate of passage. Journal of Agricultural Science (Cambridge) 92, 499-503.
Poteko, J., Schrade, S., Zeyer, K., Mohn, J., Zaehner, M., Zeitz, J.O., Kreuzer, M., Schwarm, A., 2020. Methane emissions and milk fatty acid profiles in dairy cows fed linseed, measured at the group level in a naturally ventilated housing and individually in respiration chambers. Animals (Basel) 10, 1091. doi: 10.3390/ani10061091.
Preziuso, G., Russo, C., Casarosa, L., Campodoni, G., Piloni, S., Cianci, D., 1999. Effect of diet energy source on weight gain and carcass characteristics of lambs. Small Ruminant Research 33, 9-15, doi: 10.1016/S0921- 4488(98)00202-8.
Salinas, J., Ramirez, R.G., Dominguez, M.M., Reyes-Bernal, N., Trinidad-Larraga, N., Montano, M.F., 2006. Effect of calcium soaps of tallow on growth performance and carcass characteristics of Pelibuey lambs. Small Ruminant Research 66, 135-139, doi:10.1016/j.smallrumres.2005.07.058.
SAS, 1999. SAS User’s Guide: Statistics. Version 9.1. SAS Institute Inc., Cary, North Carolina. USA.
Sato, Y., Tominaga, K., Aoki, H., Murayama, M., Oishi, K., Hirooka, H., 2020. Calcium salts of long-chain fatty acids from linseed oil decrease methane production by altering the rumen microbiome in vitro. PLoS ONE 15, e0242158. doi:10.1371/journal.pone.0242158.
Silva, M., Dehority, B.K., 2009. Ionized calcium requirement of rumen cellulolytic bacteria. Journal of Dairy Science 92, 5079-5091. doi:10.3168/jds.2009-2130.
Vandoni, S.L., Dell’Orto, V., Sgoifo Rossi, C.A., 2010. Effects of administration of three different by-pass lipids on growth performance, rumen activity and feeding behavior of beef cattle. Italian Journal of Animal Science 9, e44. 39.
van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583-3597.
Wang X, Martin GB, Wen Q, Liu S, Li Y, Shi B, Guo X, Zhao Y, Guo Y, Yan S. Palm oil protects α-linolenic acid from rumen biohydrogenation and muscle oxidation in Cashmere goat kids. Journal of Animal Science and Biotechnology 2020 11, 100. doi: 10.1186/s40104-020-00502-w.
Weld, K.A., Armentano, L.E. 2017. The effects of adding fat to diets of lactating dairy cows on total-tract neutral detergent fiber digestibility: A meta-analysis. Journal of Dairy Science 100, 1766-1779. doi: 10.3168/jds.2016-11500.