Moringa peregrina in ruminant nutrition: effects on rumen fermentation, digestion and microbial enzymes activity in vitro

Document Type : Original Research Articles (Regular Papers)

Authors

1 Department of Animal Science, Lorestan University

2 Animal Science Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Ahvaz, Iran

3 Lorestan University

10.22103/jlst.2021.17742.1371

Abstract

This study was conducted to evaluate chemical and mineral compositions, in vitro gas production (IVGP) and fermentation parameters, nutrient digestibility and rumen microbial enzymes activity of different parts of Moringa peregrina (MP) including leaves, stems and whole fodder compared to alfalfa hay (AH; four experimental treatments) using gas production (GP) technique. Leaves of MP had a higher crude protein (CP) than other fodder parts as well as AH. Content of neutral detergent fiber (NDF) in stems was higher than other experimental feed ingredients and it was comparable to AH. Highest and lowest non-fiber carbohydrates contents were observed in leaf part and AH, respectively. Regarding mineral contents, except for P and Fe which was highest in MP leaves, other minerals was highest in AH. Highest total GP and potential GP (b) was observed by stems incubation (p <0.05). However, in vitro dry matter (DM) and organic matter digestibility, metabolizable energy, microbial protein synthesis and ammonia-N concentration for MP leaves were higher than stem and AH (p <0.05). The highest and lowest DM and NDF two-stage disappearance rate was observed for MP leaves and stems, respectively (p <0.05). However, the highest and lowest carboxymethyl cellulase and microcrystalline cellulase activity were observed by incubation of MP stems and leaves, respectively (p <0.05). Activity of filter paper-degrading activity was unchanged among experimental feeds (P>0.05). Leaves of MP and alfalfa resulted in highest and lowest ruminal ɑ-amylase activity respectively (P>0.05). In conclusion, results indicated that different parts of MP fodder have potential nutritional value as alternative protein source for ruminant feeding, and leaves was also had higher nutritive value than other plant parts as well as AH.

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Main Subjects


References
Abas, I., Özpinar, H., Kutay, H.C., Kahraman, R., 2005. Determination of the metabolizable energy (ME) and net energy lactation (NEL) contents of some feeds in the Marmara region by in vitro gas technique. Turkish Journal of Veterinary and Animal Sciences 29, 751-757.
Agarwal, N., 2000. Estimation of fiber degrading enzyme. In: Chaudhary, L.C., Agarwal, N., Kamra, D.N., Agarwal, D.K., (Eds.), Feed Microbiology. CAS Animal Nutrition, IVRI, Izatnagar, India, pp. 278–291.
AOAC, 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA.
Azizi-Shotorkhoft, A., Sharifi, A., Azarfar, A., Kiani, A., 2018. Effects of different carbohydrate sources on activity of rumen microbial enzymes and nitrogen retention in sheep fed diet containing recycled poultry bedding. Journal of Applied Animal Research 46, 50-54.
Blümmel, M., Steingss, H., Becker, K., 1997. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition 77, 911–921.
Cone, J.W., van Gelder, A.H., 1999. Influence of protein fermentation on gas production profiles. Animal Feed Science and Technology 76, 251-264.
Coppin, J., 2008. A study of the nutritional and medicinal values of Moringa oleifera leaves from sub-Saharan Africa: Ghana, Rwanda, Senegal and Zambia. Master’s Thesis. New Brunswick, NJ: The State University of New Jersey, USA.
FASS, 2010. Guide for the Care and Use of Agricultural Animals in Research and Teaching. 3rd edn. Federation of Animal Science Societies, Champaign, IL.  
Foidl, N., Makkar, H.P.S., Becker, K., 2001. The potential of Moringa oleifera for agricultural and industrial uses. In: Fuglie, L.J., (Ed.). The Miracle Tree, Technical Centre for Agricultural and Rural Cooperation. Dakar (Senegal), pp. 45-76.
Getachew, G., Blummel, M., Makkar, H.P.S., Becker, K., 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Animal Feed Science and Technology 72, 261–281.
Getachew, G., Makkar, H.P.S., Becker, K., 2002. Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal of Agricultural Science 139, 341–352.
Hall, M.B., 2000. Calculation of non-structural carbohydrate content of feeds that contain non-protein nitrogen. University of Florida; Gainesville, FL, USA (Bulletin 339).
Kholif, A., Gouda, G., Morsy, T., Salem, A., Lopez, S., Kholif, A., 2015: Moringa oleifera leaf meal as a protein source in lactating goat’s diets: feed intake, digestibility, ruminal fermentation, milk yield and composition, and its fatty acids profile. Small Ruminant Research 129, 129–137.
Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J., Bertoli, S., 2015. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: an overview. International Journal of Molecular Sciences 16, 12791–12835.
Marten, G.C., Barnes, R.F., 1980. Prediction of energy digestibility of forages with in vitro rumen fermentation and fungal enzyme systems. In: Pigden, W.J., Balch, C.C., Graham, M., (Eds.). Standardization of Analytical Methodology for Feeds. Int. Devel. Res. Ctr., Ottawa, ON, Canada, pp. 61-71.
Melesse, A., 2012. The feeding value of deseeded pods from Moringa stenopetala and Moringa oleifera as evaluated by chemical analyses and in vitro gas production. Journal of Animal and Feed Sciences 21, 537–550.
Mendieta-Araica, B., Sporndly, R., Sanchez, N.R., Sporndly, E., 2011. Moringa (Moringa oleifera) leaf meal as a source of protein in locally produced concentrates for dairy cows fed low protein diets in tropical areas. Livestock Science 137, 10-17.
Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D., Schneider, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science 93, 217-222.
Miller, J.L., 1959. Modified DNS method for reducing sugars. Analytical Chemistry 31, 426–429.
Miltimore, J.E., Mason, J.L., Ashby, D.L., 1970. Copper, zinc, Manganese and Iron variation in five feeds for ruminants. Canadian Journal of Animal Science 50, 293-300.
Nasr, H., 1950. Amylolytic activity in the rumen of the sheep. Journal of Agricultural Science 40, 308–310.
Negesse, T., Makkar, H.P.S., Becker, K., 2009. Nutritive value of some non-conventional feed resources of Ethiopia determined by chemical analyses and an in vitro gas method. Animal Feed Science and Techology 154, 204-217
Newton, K.A., Bennett, R.N., Curto, R.B.L., Rosa, E.A.S., Turc, V.L., Giuffrida, A., Curto, A.L., Crea, F., Timpo, G.M., 2010. Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana. Food Chemistry 122, 1047–1064.
Nogueira Filho, J.C.M., Fondevila, M., Barrios Urdaneta, A., Gonzalez Ronquillo, M., 2000. In vitro microbial fermentation of tropical grasses at an advanced maturity stage. Animal Feed Science and Technology 83, 145-157.
Nouman, W., Basra, S.M.A., Siddiqui, M.T., Yasmeen, A., Gull, T., Alcayde, M.A.C., 2014. Potential of Moringa oleifera L. as livestock fodder crop: a review. Turkish Journal of Agriculture and Forestry 38, 1–14.
NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th Revised ed. National Research Council,  National Academy of Sciences, National Academy Press, Washington, D.C.
Ørskov, E.R., McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. Journal of Agricultural Science 92, 499–503.
Pashaei, S., Razmazar, V., Mirshekar, R., 2010. Gas Production: a proposed in vitro method to estimate the extent of digestion of a feedstuff in the rumen. Journal of Biological Sciences 10, 573-580.
Robertson, J.B., Van Soest, P.J., 1981. The detergent system of analysis and its application to human foods. In: James, W.P.T., Theander, O. (Eds.), The Analysis of Dietary Fiber in Food. Marcel Dekker, NY, USA, pp. 123–158.
Saalu, L.C., Osinubi, A.A., Akinbami, A.A., Yama, O.E., Oyewopo, A.O., Enaibe, B.U., 2011. Moringa oleifera Lamarck (drumstick) leaf extract modulates the evidences of hydroxyureainduced testicular derangement. International Journal of Applied Research in Natural Products 4, 32–45.
Salem, A.Z.M., 2012. Oral administration of leaf extracts to rumen liquid donor lambs modifies in vitro gas production of other tree leaves. Animal Feed Science and Technology 176, 94-101.
Sallam, S.M.A., Buenob, I.C.S., Godoyb, P.B., Nozellab, E.F., Vittib, D.M.S.S., Abdallab, A.L., 2008. Nutritive value assessment of artichoke (Cynara scolymus) by-products as an alternative feed resource for ruminants. Tropical and Subtropical Agroecosystems 8, 181-189.
Sanchez, N.R., Stig, L., Inger, L., 2006. Biomass production and chemical composition of Moringa oleifera under different management regimes in Nicaragua. Agroforestry Systems 66, 231–242.
SAS, 2002. SAS User’s Guide: Statistics. Statistical Analysis Systems Institute Inc., Cary, North Carolina. USA
Soliva, C.R., Kreuzer, M., Foidl, N., Foidl, G., Machmuller, A., Hess, H.D., 2005. Feeding value of whole and extracted Moringa oleifera leaves for ruminants and their effects on ruminal fermentation in vitro. Animal Feed Science and Technology 118, 47–62.
Sun, B., Zhang, Y., Ding, M., Xi, Q., Liu, G., Li, Y., Liu, D., Chen, X., 2018. Effects of Moringa oleifera leaves as a substitute for alfalfa meal on nutrient digestibility, growth performance, carcass trait, meat quality, antioxidant capacity and biochemical parameters of rabbits. Journal of Animal Physiology and Animal Nutrition 102, 194–203.
Teixeira, E.M.B., Carvalho, M.R.B., Neves, V.A., Silva, M.A., Arantes-Pereira, L., 2014. Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. leaves. Food Chemistry 147, 51–54.
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B.,  France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48, 185–197.
Tilley, J.M.A., Terry, R.A., 1963. A two stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society 18, 104–111.
Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
Zeng, B., Sun, J.J., Chen, T., Sun, B.L., He, Q., Chen, X.Y., Zhang, Y.L., Xi, Q.Y., 2018. Effects of Moringa oleifera silage on milk yield, nutrient digestibility and serum biochemical indexes of lactating dairy cows. Journal of Animal Physiology and Animal Nutrition 102, 75–81.