Effects of holding semen for 4 h at 15 °C in different extenders prior to cryopreservation on sperm characteristics and subpopulation kinematics in INRA180 sheep

Document Type : Research Article (Regular Paper)

Authors

1 INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco

2 INRA, Division scientifique, Département des productions animales, Rabat

3 Institut National de la Recherche Agronomique, Centre Régional de Settat

Abstract

The aim of the present study was to evaluate the effects of holding semen for 4 h at 15 °C in different extenders prior to cryopreservation on sperm characteristics and subpopulation kinematics in INRA180 sheep. Semen samples were collected using an artificial vagina from two animal groups. Group 1: the rams kept next to the lab (G1) considered as a control and group 2: the rams kept in a farm faraway (170 km) from the lab (G2). The semen was extended in Milk egg yolk, Andromed® and Tryladil® extenders to reach 0.2 x 109 spermatozoa/mL. Before freezing, the samples from G1 were directly cooled to 5 °C during 2 h. While those for G2 were transported within 4 h to the laboratory in a temperature-regulated cooler box at 15 °C then cooled to 5 °C. Differences in sperm motility, viability and abnormality in pre- and post-thaw steps and subpopulation kinetic parameters in post thaw step were detected between the two groups, and between extenders within the groups. Evaluation of the kinetic data obtained with a CASA (Computer-Assisted Sperm Analysis), applying a cluster analysis, yielded in INRA180 rams two post thaw sperm subpopulations (SPs). The SP1 and SP2 included spermatozoa characterized by low and high kinetic parameters, respectively. Disregarding the extender type, the proportional size of SP1 was lower than SP2 in G1, Nevertheless, in G2 the percentage of SP1 was higher than SP2. In conclusion, changing conditions during the equilibration time (from 2 h at 5 °C to 4 h at 15 °C) can extend the total process duration and causes a decrease in post-thawed semen quality in INRA180 rams.

Keywords

Main Subjects

References

  • Aires, V.A., Hinsch, K.D., Mueller-Schloesser, F., Bogner, K., Mueller-Schloesser, S., Hinsch, E., 2003. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology 60, 269–279.
  • Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gérard, O., Courtens, J.L., Anton, M., 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl, a commercial egg yolk extender. Theriogenology 61, 895–907.
  • Backman, T., Bruemmer, J.E., Graham, J.K., Squires, E.L., 2004. Pregnancy rates of mares inseminated with semen cooled for 18 hours and then frozen. Journal of Animal Science 82, 690–694.
  • Benmoula, A., Allai, L., Badi, A., Khalil, K.E., Amiri, B.E., 2018. Effect of extender and storage temperature on sperm motility parameters of liquid ram semen Revue Marocaine des Sciences Agronomiques et Vétérinaires 6, 211-219.
  • Colas, G., 1975. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen. Journal of Reproduction and Fertility 42, 277–285.
  • Contri, A., Zambelli, D., Faustini, M., Cunto, M., Gloria, A., Carluccio, A., 2012. Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat. Reproduction 144, 339–347. https://doi.org/10.1530/REP-12-0125
  • Dorado, J., Molina, I., Muñoz-Serrano, A., Hidalgo, M., 2010. Identification of sperm subpopulations with defined motility characteristics in ejaculates from Florida goats. Theriogenology 74, 795–804. https://doi.org/10.1016/j.theriogenology.2010.04.004
  • Drobnis, E.Z., Crowe, L.M., Berger, T., Anchordoguy, T.J., Overstreet, J.W., Crowe, J.H., 1993. Cold shock damage is due to lipid phase transitions in cell membranes: a demonstration using sperm as a model. Journal of Experimental Zoology 265, 432–437. https://doi.org/10.1002/jez.1402650413
  • Eriksson, B.M., Vazquez, J.M., Martinez, E.A., Roca, J., Lucas, X., Rodriguez-Martinez, H., 2001. Effects of holding time during cooling and of type of package on plasma membrane integrity, motility and in vitro oocyte penetration ability of frozen-thawed boar spermatozoa. Theriogenology 55, 1593–1605.
  • Evans, G., Maxwell, W.M.C., Salamon, S., 1987. Salamon’s Artificial Insemination of Sheep and Goats. Butterworths, Sydney.
  • Foote, R.H., Kaprotht, M.T., 2002. Large batch freezing of bull semen: effect of time of freezing and fructose on fertility. Journal of  Dairy Science 85, 453–456.
  • Forouzanfar, M., Sharafi, M., Hosseini, S.M., Ostadhosseini, S., Hajian, M., Hosseini, L., Abedi, P., Nili, N., Rahmani, H.R., Nasr-Esfahani, M.H., 2010. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 73, 480–487.
  • Fukui, Y., Kohno, H., Togari, T., Hiwasa, M., Okabe, K., 2008. Fertility after artificial insemination using a soybean-based semen extender in sheep. Journal of Reproduction and Development 54, 286–289.
  • Gil, J., Lundeheim, N., Söderquist, L., Rodriíuez-Martínez, H., 2003. Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology 59, 1241–1255.
  • Graham, E.F., Erickson, W.E., Bayley, N.D., 1957. Effect of Glycerol Equilibration on Frozen Bovine Spermatozoa1. Journal of  Dairy Science 40, 510–515.
  • Guthrie, H.D., Welch, G.R., 2005. Impact of storage prior to cryopreservation on plasma membrane function and fertility of boar sperm. Theriogenology 63, 396–410.
  • Holt, W.V., O’Brien, J., Abaigar, T., 2007. Applications and interpretation of computer-assisted sperm analyses and sperm sorting methods in assisted breeding and comparative research. Reproduction, Fertility and  Development 19, 709–718.
  • Kanuga, M.K., Drew, R.E., Wilson-Leedy, J.G., Ingermann, R.L., 2012. Subpopulation distribution of motile sperm relative to activation medium in steelhead (Oncorhynchus mykiss). Theriogenology 77, 916–925.
  • Leboeuf, B., Restall, B., Salamon, S., 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science 62, 113–141.
  • Ledesma, A., Zalazar, L., Fernández-Alegre, E., Hozbor, F., Cesari, A., Martínez-Pastor, F., 2017. Seminal plasma proteins modify the distribution of sperm subpopulations in cryopreserved semen of rams with lesser fertility. Animal Reproduction Science 184, 44–50.
  • Martínez-Pastor, F., Tizado, E.J., Garde, J.J., Anel, L., de Paz, P., 2011. Statistical Series: Opportunities and challenges of sperm motility subpopulation analysis. Theriogenology 75, 783–795.
  • Murphy, E.M., Eivers, B., O’Meara, C.M., Lonergan, P., Fair, S., 2018. Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination. Theriogenology 108,
  • Nur, Z., Zik, B., Ustuner, B., Sagirkaya, H., Ozguden, C.G., 2010. Effects of different cryoprotective agents on ram sperm morphology and DNAintegrity. Theriogenology 73, 1267–1275.
  • Peris, S.I., Morrier, A., Dufour, M., Bailey, J.L., 2004. Cryopreservation of ram semen facilitates sperm DNA damage: relationship between sperm andrological parameters and the sperm chromatin structure assay. Journal of Andrology 25, 224–233.
  • Purdy, P.H., Mocé, E., Stobart, R., Murdoch, W.J., Moss, G.E., Larson, B., Ramsey, S., Graham, J.K., Blackburn, H.D., 2010. The fertility of ram sperm held for 24h at 5 degrees C prior to cryopreservation. Animal Reproduction Science 118, 231–235.
  • Quintero-Moreno, A., Miró, J., Teresa Rigau, A., Rodríguez-Gil, J.E., 2003. Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenology 59, 1973–1990.
  • Salamon, S., Maxwell, W.M., 2000. Storage of ram semen. Animal Reproduction Science 62, 77–111.
  • Soylu, M.K., Nur, Z., Ustuner, B., Dogan, I., Sagirkaya, H., Gunay, U., Ak, K., 2007. Effects of various cryoprotective agents and extender osmolality on post-thawed ram semen. Bulletin-Veterinary Institute In Pulawy 51, 241-246.
  • Torres, M.A., Monteiro, M.S., Passarelli, M.S., Papa, F.O., Dell’Aqua, J.A., Alvarenga, M.A., Martins, S.M.M.K., de Andrade, A.F.C., 2019. The ideal holding time for boar semen is 24 h at 17 °C prior to short-cryopreservation protocols. Cryobiology 86, 58-64.
  • Üstüner, B., Alçay, S., Nur, Z., Sağİrkaya, H., Soylu, M.K., 2014. Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Derg 20, 393–398.
  • van der Horst, G., Maree, L., du Plessis, S.S., 2018. Current perspectives of CASA applications in diverse mammalian spermatozoa. Reproduction, Fertility and Development 30, 875–888.
  • Vichas, L., Tsakmakidis, I.A., Vafiadis, D., Tsousis, G., Malama, E., Boscos, C.M., 2018. The effect of antioxidant agents’ addition and freezing method on quality parameters of frozen thawed ram semen. Cell Tissue Bank 19, 113–121.
  • Watson, P.F., 2000. The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science 60–61, 481–492.
  • Zhang, S., Hu, J., Li, Q., Jiang, Z., Zhang, X., 2009. The cryoprotective effects of soybean lecithin on boar spermatozoa quality. African Journal of  Biotechnology 8, 6476-6480
Journal of Livestock Science and Technologies
Volume 7, Issue 2
December 2019
Pages 65-75

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