The purpose of this study was to investigate the effect of processed alfalfa silage with acetic acid and Ecosyl on feed intake, microbial protein synthesis and blood parameters in four Moghani male sheep (90±2 kg BW) using a changeover design consisting of four 14-day periods. The experimental treatments were 1) alfalfa silage without additive as control, 2) alfalfa silage treated with Ecosyl, 3) alfalfa silage treated with 5% acetic acid, and 4) alfalfa silage treated with acetic acid and Ecosyl. The results showed that feed intake was not affected by treatments. The amounts of excreted purine derivatives were 12.29, 7.02, 12.03 and 8.64 g/d for alfalfa silage (control), alfalfa silage treated with acetic acid or Ecosyl and their combination. Also, the adsorbed purine derivatives values were 13.46, 7.19, 13.14 and 9.12 g/d for control, alfalfa silage treated with acetic acid or Ecosyl and their combination. Untreated alfalfa silage, treated with either acetic acid, Ecosyl or their combination resulted in 9.79, 5.23, 9.56 and 6.63 g/d of microbial protein synthesis, respectively. The excreted purine and adsorbed purine derivatives and microbial protein synthesis were reduced in alfalfa silage treated with acetic acid and Ecosyl and their combination (P<0.05). There were no significant differences in blood metabolites among the treatments, except for cholesterol values. Alfalfa silage treated with acetic acid alone did not change the nutritional values; however, alfalfa silage treated with acetic acid and Ecosyl improved the nutritional parameters in sheep.
Adesogan, A.T., Kim, S.C., Arriola, K.G., Dean, D.B., Staples, C.R., 2007. Strategic addition of dietary fibrolytic enzymes for improved performance of lactating dairy cows. Proceedings of the 18th Annual Florida Ruminant Nutrition Symposium, Gainesville, USA.
Adesogan, A.T., Salawu, M., 2004. Effect of applying formic acid, heterolactic bacteria or homolactic and heterolactic bacteria on the fermentation of bi-crops of peas and wheat. Journal of the Science of Food and Agriculture 84, 983 - 992.
Agarwal, N., Kamra, D.N., Chaudhary, L.C., Sahoo, A., Pathak, N.N., 2002. Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 34, 329-36.
Aksu, T., Denek, N., Aydin, Ss., Doğan, Daş., Savrunlu, M., Özkaya, S., 2017. Effect of dried thyme pulp (Tymbra spicata L. spicata) on fermantation quality and in vitro organic matter digestibility of meadow grass and alfalfa silages. Kafkas Universitesi Veteriner Fakultesi Dergisi 23, 211-217.
Ali, A., Sarzamin, K., Muhammad, M., Muhammad, I., Iftikhar, A., Khan, A.N., Mubarak, A., Hamayun, K., 2013. Effect of different levels of organic acids supplementation on feed intake, milk yield and milk composition of dairy cows during thermal stress. Greener Journal of Agricultural Sciences 3, 762-768.
AOAC, 2000. Official Methods of Analysis. 17th ed. Association of Official Analytical Chemists, Washington, DC. USA.
Balcells, J., Guada, J.A., Peiro, J.M., Parker, D.S., 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high performance liquid chromatography. Journal of Chromatography 575, 153-157.
Behgar, M., Danesh-Mesgaran, M., Nasiri Moghaddam, H., Sobhani Rad, S., 2007. Chemical composition, dry matter and crude protein degradability of alfalfa silage treated with formic and sulphuric acids and its effect on performance of early lactating Holstein cows. Journal of Science and Technology of Agriculture and Natural Resources11, 339-349. (In Persian).
Cannas, A., Tedeschi, L.O., Fox, D.G., Pell, A.N., Van Soest, P.J., 2004. A mechanistic model for predicting the nutrient requirements and feed biological values for sheep. Journal of Animal Science 82, 149–169.
Chaudhary, L.C., Sahoo, A., Agrawal, N., Kamra, D.N., Pathak, N.N., 2008. Effect of direct fed microbial on nutrient utilization, rumen fermentation, immune and growth response in crossbred cattle calves. IndianJournal ofAnimal Sciences 78, 515-521.
Chen, L., Guo, G., Yuan, X., Shimojo, M., Yu, C., Shao, T., 2014. Effect of applying molasses and propionic acid on fermentation quality and aerobic stability of total mixed ration silage prepared with whole-plant corn in Tibet. Journal of Dairy Science 27, 349-356.
Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., Yuan, Z., 2014. Antibiotics alternatives: the substitution of antibiotics in animal husbandry? Frontiers in Microbiology 5, 217.
Chen, X.B., Gomes, M.J., 1995. Estimation of Microbial Protein Supply to Sheep and Cattle Based on Urinary Excretion of Purine Derivatives. International Feed Resources Unit, Rowett Research Institute, Aberdeen, UK.
Chen, X.B., Hovell, F.D., Ørskov, E.R., Brown, D.S., 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131-142.
Chen, X.B., Øreskov, E.R., 2004. Research on Urinary Excretion of Purine Derivatives in Ruminants: Past, Present and Future, International Feed Resources Unit, Macaulary Land Research Institute, Aberdeen, UK.
Church, D.C., Pond, W.G., 1988. Basic Animal Nutrition and Feeding. 3rd ed. Wiley, New York.
Delaware, M.H., Danesh-Mesgaran, M., 2003. Chemical and gastrointestinal components (rumen and intestine) of sulfuric urea silage treated alfalfa and its effect on its production and production in lactating milk. Journal of Water and Soil (Agricultural Sciences and Technology) 17, 219 -231. (In Persian).
Donovan, D.C., Franklin, S.T., Chase, C.C., Hippen, A.R., 2002. Growth and health of Holstein calves fed replacer supplemented with antibiotics or Enteroguard. Journal of Dairy Science 85, 947-950.
Eun, J.S., Fellner, V., Burns, J.C., Gumpertz, M.L., 2004. Fermentation of eastern gamagrass (Tripsacum dactyloides) by mixed cultures of ruminal microorganisms with or without supplemental corn. Journal of Animal Science 82, 170–178.
Fabiszewska, A.U., Zielińska, K.J., Wróbel, B., 2019. Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a mini review. World Journal of Microbiology and Biotechnology 35, 1-8.
Galvao, K.N., Santos, J.E., Coscioni, A., Villasenor, M., Sischo, W.M., Berge, A.C., 2005. Effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal Escherichia coli. Reproduction Nutrition Development 45, 427-440.
Ghodousi, M., Diani, A., Khazri, A., Sharifi Hosseini, M., 2015. The effect of different levels of banana (cut branches with leaf) silage with waste date on feed intake, microbial protein synthesis and sheep blood parameters. Journal ofAnimal Production 17, 359-370. (In Persian).
Guo, X.S., Ke, W.C., Ding, W.R., Ding, L.M., Xu, D.M., Wang, W.W., Zhang, P., Yang, F.Y., 2018. Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri. Scientific Reports 8, 357–366.
Hajalizadeh, Z., Dayani, A., Tahmasebi, R., Khazri, A., 2013. Evaluation of chemical composition of pistachio pulp silage and its effect on feed intake, rumen fermentation characteristics and blood parameters in sheep. Journal of Animal Science Researches 24, 82-94. (In Persian).
Hossein-Abadi, M., Dehghan-Benadaki, M., Zali, A., 2013. Effect of bacterial probiotic supplementation on raw milk or feed on growth performance, health status, blood parameters and rumen of Holstein calves. Animal Production Research 4, 57-69. (In Persian).
Karmshahi, K., Dayani, O., Tahmasebi, R., Khazri, A., 2014. Effect of feeding Alhagi with waste date palm silage on rumen parameters and microbial protein synthesis in sheep. IranianJournal of Animal Science 45, 257-271. (In Persian).
Ke, W.C., Ding, W.R., Xu, D.M., Ding, L.M., Zhang, P., Li, F.D., Guo, X.S., 2017. Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage. Journal of Dairy Science 100, 8958-8966.
Khorvash, M., Mohammadzadeh, H., Bahrami, M., 2014. Production and Consumption Management of Silage. 1st ed. Isfahan. Arkan Danesh Publications, Iran. (In Persian).
Kung, JR, L., Tung R.S., Maciorowski, K., 1991. Effect of a microbial inoculant (Ecosyl™ and/or a glycopeptide antibiotic (vancomycin) on fermentation and aerobic stability of wilted alfalfa silage. Animal Feed Science and Technology 35, 37-48.
Kung, JR, L., Shaver, R.D., Grant, R.J., Schmidt R.J., 2018. Silage review: interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science 101, 4020 - 4033.
Li, P., Ji, S., Hou, C., Tang, H., Wang, Q., Shen, Y., 2016. Effects of chemical additives on the fermentation quality and N distribution of alfalfa silage in south of China. Animal Science Journal 87, 1472–1479.
McDonald, P., Henderson, A.R., Heron, S.J.E., 1991. The Biochemistry of Silage. 2nd ed. Chalcombe Publications, Marlow Bottom, Marlow, Bucks, UK.
Mohammadi Roodposhti, P., Dabiri, N., 2012. Effects of probiotic and prebiotic on average daily gain, fecal shedding of Escherichia coli and immune system status in newborn female calves. Asian Australasian Journal of Animal Sciences 25, 1255-1261.
Nadeau, E.M.G., Boxton, D.R., Linogreh, E., Lingvall, P., 1996. Kinetics of cell-wall digestion of orchardgrass and alfalfa silage treated with cellulase and formic acid. Journal of Animal Science 79, 2207-2216.
Nisa, M.U., Tauqir, N.A., Sarwar, M., Khan, M.A., Akhtar, M., 2005. Effect of additives and fermentation periods on chemical composition and in situ digestion kinetics of mott grass (Pennisetum purpureum) silage. Asian-Australasian Journal of Animal Sciences 18, 812–815.
Ogunade, I.M., Jiang, Y., Pech Cervantes, A.A., Kim, D.H., Oliveira, A.S., Vyas, D., Weinberg, Z.G., Jeong, K.C., Adesogan, A.T., 2018. Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives. Journal of Dairy Science 101, 2048-2059.
Riddell, J.B., Gallegos, A.J., Harmon, D.L., Mcleod, K.R., 2010. Addition of a Bacillus based probiotic to the diet of pre ruminant calves: influence on growth, health, and blood parameters. International Journal of Applied Research in Veterinary Medicine 8, 78-85.
Rizk, C., Mustafa, A.F., Phillip, L.E., 2005. Effects of inoculation of high dry matter alfalfa silage on ensiling characteristics, ruminal nutrient degradability and dairy cow performance. Journal of the Science of Food and Agriculture 85,743-750.
Sadeghi, K., Khorvash, M., Ghorbani, G.R., Forouzmand, M.A., Boroumand, M., Hashemzadeh-Cigari, F., 2012. Effects of homo-fermentative bacterial inoculants on fermentation characteristics and nutritive value of low dry matter corn silage. Iranian Journal of Veterinary Research 13, 303-309.
SAS, 2003. SAS/STAT Software: Changes and Enhances Through Release 9.1.3. SAS Institute Inc., Cary, North Carolina. USA.
Shakeri, P., Riaasi, A., Alikhani, M., Ghorbani, Gr., Fazaeli, H., 2011. Effects of nutrition of pistachio by-product silage on microbial protein synthesis and kidney function in Holstein male calves. Journal Animal ScienceResearch 21, 97-110. (In Persian).
Sifeeldein, A., Yuan, X., Dong, Z., Li, J., Shao, T., 2018. Effect of applying Lactobacillus plantarum and Pediococcus acidilactici Isolated on fermentation dynamics, microbial community and aerobic stability of Napier grass (Pennisetum purpureum) silage. Kafkas Universitesi Veteriner Fakultesi Dergisi 24, 371-378.
Sifeeldein, A., Yuan, X., Dong, Z., Li, J., Youns, H., Shao, T., 2018. Characterization and identification of lactic acid bacteria by 16S rRNA gene sequence and their effect on the fermentation quality of Elephant grass (Pennisetum purpureum) silage. Kafkas Universitesi Veteriner Fakultesi Dergisi 24, 123-130.
Silva, V.P., Pereira, O.G., Leandro, E.S., Da Silva, T.C., Ribeiro, K.G., Mantovani, H.C., Santos, S.A., 2016. Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions. Journal of Dairy Science 99, 1895-1902.
Tedeschi, L.O., Cannas, A., Fox, D.G., 2010. A nutrition mathematical model to account for dietary supply and requirements of energy and other nutrients for domesticated small ruminants: The development and evaluation of the Small Ruminant Nutrition System. Small Ruminant Research 89,174–184.
Tamminga, S., Ketelaar, R., Van Vuuren, A.M., 1991. Degradation of nitrogenous compounds in conserved forages in the rumen of dairy cows. Grass ForageScience 46, 427-435.
Vakil-Faraji, Y., Jafari-Khorshidi, K., Zahedi-Far, M., 2009. The effect of different level of concentrate in diet on microbial protein synthesis in native Mazandaran province buffalo rumen. Journal of Large Animal Clinical Science Research (Journal of Veterinary Medicine) 7, 61-66.
Valadares, R.F., Broderick, G.A., Valadares Filho, S.C., Clayton, M.K., 1999. Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives. Journal of Dairy Science 82, 2686–2696.
Van Soest, P.J., 1994. Nutritional Ecology of the Ruminant, 2nd ed. Comstock Publishing Associates/Cornell University Press, Ithaca, NY, USA.
Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583- 3597.
Verbic, J., Orskov, E.R., Zgajnar, J., Chen, X.B., Zindrsic-Pongrac, V., 1999. The effect of method of forage preservation on the protein synthesis in the rumen. Animal Feed Science and Technology 82, 195-212.
Weinberg, Z.G., Muck, R.E., Weimer, P.J., Chen, Y., Gamburg, M., 2004. Lactic acid bacteria used in silage inoculants as probiotics for ruminants. Applied Biochemistry and Biotechnology 118, 1–10.
Whiter, A.G., Kung, Jr, L., 2001. The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of alfalfa silage. Journal of Dairy Science 84, 2195–2202.
Yu, P., Eng, A.R., Boon-ek, L., Leury, B.J., 2002. Purine derivative excretion and ruminal microbial yield in growing lambs fed raw a dry roasted legume seeds as protein supplemented. Journal Animal Feed Science Technology 95, 33-48.
Zamani, Z., Ali Arabi, H., Tabatabai, M.M., Zamani, P., Saki, A.S., Zaboli, Kh., 2008. Effect of different levels of urea and sulfur on microbial protein synthesis, nitrogen retention, some blood metabolites and nutrients digestibility in Mehraban sheep. Agricultural Research 7, 71-83. (In Persian).
Zielińska, K., Fabiszewska, A., Stefańska, I., 2015. Diferent aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage. Chilean Journal of Agricultural Research 75, 298–306.
Barzegar Mashak, R., Seifdavati, J., Abdi-Benemar, H., Mirzaei Aghjehgheshlagh, F., Seyedsharifi, R., & Yalchi, T. (2019). The effect of alfalfa silage treated with Ecosyl and acetic acid on microbial protein synthesis and blood parameters in Moghani male sheep. Journal of Livestock Science and Technologies, 7(2), 37-45. doi: 10.22103/jlst.2020.14635.1288
MLA
Roghaie Barzegar Mashak; Jamal Seifdavati; Hossein Abdi-Benemar; Farzad Mirzaei Aghjehgheshlagh; Reza Seyedsharifi; Taher Yalchi. "The effect of alfalfa silage treated with Ecosyl and acetic acid on microbial protein synthesis and blood parameters in Moghani male sheep", Journal of Livestock Science and Technologies, 7, 2, 2019, 37-45. doi: 10.22103/jlst.2020.14635.1288
HARVARD
Barzegar Mashak, R., Seifdavati, J., Abdi-Benemar, H., Mirzaei Aghjehgheshlagh, F., Seyedsharifi, R., Yalchi, T. (2019). 'The effect of alfalfa silage treated with Ecosyl and acetic acid on microbial protein synthesis and blood parameters in Moghani male sheep', Journal of Livestock Science and Technologies, 7(2), pp. 37-45. doi: 10.22103/jlst.2020.14635.1288
VANCOUVER
Barzegar Mashak, R., Seifdavati, J., Abdi-Benemar, H., Mirzaei Aghjehgheshlagh, F., Seyedsharifi, R., Yalchi, T. The effect of alfalfa silage treated with Ecosyl and acetic acid on microbial protein synthesis and blood parameters in Moghani male sheep. Journal of Livestock Science and Technologies, 2019; 7(2): 37-45. doi: 10.22103/jlst.2020.14635.1288
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