The effects of feeding alfalfa pulp ensiled with wasted date (Phoenix dactylifera L.) on digestibility, microbial protein synthesis and ruminal fermentation characteristics in sheep

Document Type : Original Research Article (Regular Paper)

Authors

1 Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran.

2 Department of Animal Science, Ilam Agricultural and Natural Resources Research and Education Center, AREEO, Ilam, Iran.

Abstract

The aim of this study was to evaluate the effect of feeding alfalfa pulp ensiled with waste date (Phoenix dactylifera L.) on digestibility, microbial protein synthesis and ruminal fermentation characteristics in Kermani sheep. Alfalfa (Medicago sativa L.) pulps were ensiled with waste date (15% in dry matter) in buckets. After 45 days, chemical composition and pH of the silage were evaluated using four Kermani rams in a change-over design with four 21-day periods comprising of 16 days for adaptation and 5 days for sample collection. Treatments containing 4 diets: 1) control diet (without silage); 2) diet containing 10% silage; 3) diet containing 20% silage and 4) diet containing 30% silage. The results of this study showed that adding 15% waste date to alfalfa pulp during ensiling, improved silage quality and DM. The Flieg point and pH of silage were 94.26 and 3.8, respectively, with a total score quality evaluation of 19 that seemed to be a very good score. Nutrient digestibility, nitrogen (N) retention, blood parameters, urinary purine derivatives and microbial protein synthesis were not affected by treatments. The total population of Entodinium and total protozoa species were increased linearly with the increase in the level of alfalfa pulp ensiled with waste date in the diets. In conclusion, ensiling of alfalfa pulp with 15% waste date increased DM and silage quality and its feeding to sheep did not have negative effects on feed intake and nutrient digestibility. Due to the relatively low costs of alfalfa pulp and waste date, their inclusion in sheep diets can reduce the cost of feed and environmental pollution.

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Main Subjects


  • Alhomidy, S.N., Basmaeil, S.A.N., Al-Owaimer, A.M., El-Wazirand, M., 2011. Effect of feeding different amounts of discarded dates on growth and efficiency of digestion in sheep. Australian Journal of Basic and Applied Sciences 5, 636-640.
  • Al-Hooti, S., Sidhu, J.S., Qabazard, H., 1997. Physicochemical characteristics of five date fruit cultivars grown in the United Arab Emirates. Plant Foods for Human Nutrition 50, 101–113.
  • Allen, M.S., 2000. Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83, 1598-1624.
  • AOAC, 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Arlington, VA, USA.
  • Azizi-Shotorkhoft, A., Rezaei, J., Fazaeli, H., 2013. The effect of different levels molasses on the digestibility, rumen parameters and blood metabolites in sheep fed processed broiler litter. Animal Feed Science and Technology 179, 69-76.
  • Borreani, G., Tabacco, E., Schmidt, R.J., Holmes, B.J., Muck, R.E., 2018. Silage review: Factors affecting dry matter and quality losses in silages. Journal of Dairy Science 101, 3952-3979.
  • Brito, A.F., Broderick, G.A., 2006. Effect of varying dietary ratios of alfalfa silage to corn silage on production and nitrogen utilization in lactating dairy cows. Journal of Dairy Science 89, 3924–3938.
  • Broderick, G.A., 1995. Performance of lactation dairy cow fed either alfalfa silage or alfalfa hay as the sole forage. Journal of Dairy Science 75, 320-329.
  • Broderick, G.A., Muck, R.E., 2009. Effect of alfalfa silage storage structure and rumen-protected methionine on production in lactating dairy cows. Journal of Dairy Science 92, 1281–1289.
  • Castillo, A.R., Kebreab, E., Beever, D.E., Barbi, J.H., Sutton, J.D., Kirby, H.C., France, J., 2001. The effect of protein supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets. Journal of Animal Science 79, 247-252.
  • Chaudhry, S., Naseer, M.Z., 2006. Silage of citrus pulp-poultry Litter-Corn forage for sheep. Pakistan Journal of Agricultural Science 43, 173-179.
  • Chen, X.B., Chowdhury, S.A., Hovell, F.D., Orskov, E.R., Kyle, D.J., 1992. Endogenous allantoin excretion in response to changes in protein supply in sheep. Journal of Nutrition 122, 2226– 2232.
  • Chen, X.B., Orskov, E.R., 2004. Research on urinary excretion of purine derivatives in ruminants. Past, present and future, estimation of microbial protein supply in ruminants using urinary purine derivatives. Kluwer Academic Publisher 7, 180-212.
  • Coblentz, W.K., Grabber, J.H., 2013. In situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration. Journal of Dairy Science 96, 3120–3137.
  • Coblentz, W.K., Muck, R.E., 2012. Effects of natural and simulated rainfall on indicators of ensilability and nutritive value for wilting alfalfa forages sampled before preservation as silage. Journal of Dairy Science 95, 6635-6653.
  • Dayani, O., Ghorbani, G., Alikhani, M., Rahmani, H., Mir, P., 2007. Effects of dietary whole cottonseed and crude protein level on rumen protozoal population and fermentation parameters. Small Ruminant Research 69, 36–45.
  • Dehority, B.A., 2005. Effect of pH on viability of Entodinium caudatum, Entodinium exiguum, Epidinium caudatum, and Ophryoscolex purkynjei in vitro. Journal of Eukaryotic Microbiology 52, 339-342.
  • Depeters, E.J., Ferguson, J.D., 1992. Nonprotein nitrogen and protein distribution in the milk of cows. Journal of Dairy Science 75, 3192-3209.
  • Douglas, M., Veira, D.M., 1987. The role of ciliate protozoa in nutrition of the ruminant. Journal of Animal Science 63, 1547- 1560.
  • FAO, 2016. Production of top 5 producers. Food and Agricultural Organization of the United Nations. Statistics Division. Vol. 2016/3/4.
  • Franzolin, R., Dehority, B.A., 1996. Effect of prolonged high-concentrate feeding on ruminal protozoa concentrations. Journal of Animal Science 74, 2803-2809.
  • Gomes, M.J., Guedes, C.M., Silva, S.R., Azevedoa, J.M.T., Dias-da-Silva, A., 2014. Utilization of high and low roughage diets by a local and an exotic breed of sheep: intake, growth and digestive efficiency. Livestock Science 167, 110-120.
  • Guney, M., Demirel, M., Celik, S., Yunus, B., Taner, L., 2007. Effects of urea, molasses and urea plus molasses supplementation to sorghum silage on the silage quality, in vitro organic matter digestibility and metabolic energy contents. Journal of Biology Science 7, 401-404.
  • Hall, M.B., Herejk, C., 2001. Differences in yields of microbial crude protein from in vitro fermentation of carbohydrates. Journal of Dairy Science 48, 2486-2493.
  • Hartinger, T., Gresner, N., Sudekum, K.H., 2019. Effect of wilting intensity, dry matter content and sugar addition on nitrogen fractions in Lucerne silages. Agriculture 9, 1-17.
  • Hristov, A.N., Jouany, J.P., 2005. Factors affecting the efficiency of nitrogen utilization in the rumen. In: Hristov, A.N. (Ed.), Nitrogen and Phosphorus Nutrition of Cattle. CAB International, Cambridge, pp. 117–166.
  • Ivan, M., Neill, L., Entz, T., 2000. Ruminal fermentation and duodenal flow following progressive inoculations of fauna free withers with major individual species of ciliate protozoa or total fauna. Journal of Animal Science 78, 750-759.
  • Jakyeom, S., Jiyoung, Y., Hyun, J.K., Santi, D.U., Chol, W.M., Jong, K., 2010. Effect of synchronization of carbohydrate and protein supply on ruminal fermentation, nitrogen metabolism and microbial protein synthesis in Holstein steers. Asian-Australasian Journal of Animal Sciences 23, 1455-1461.
  • Jian, G., Cuijun, Y., Guihe, L., 2015. Nutritional evaluation of fresh and wilted mixed silage of naked oats (Avena nuda) and alfalfa (Medicago sativa). International Journal of Agriculture and Biology 17, 761-766.
  • Johnson, L.M., Harrison, J.H., Riley, R.E., 1998. Estimation of the flow of microbial nitrogen to the duodenum using urinary uric acid or allantoin. Journal of Dairy Science 81, 2408–2420.
  • Khalili, H., Huhtanen, P., 2002. Effect of casein infusion in the rumen, duodenum or both sites on factors affecting forage intake and performance of dairy cows fed red clover-grass silage. Journal of Dairy Science 85, 909-918.
  • Khezri, A., Dayani, O., Tahmasbi, R., 2016. Effect of increasing levels of wasted date palm on digestion, rumen fermentation and microbial protein synthesis in sheep. Journal of Animal Physiology and Animal Nutrition 101, 53-60.
  • Kilic, A., 1984. Silage Feed. Bilgehan Press, Izmir, Turkey, pp. 350.
  • Kilic, A., 1986. Silo feed. Instruction, Education and Application Proposals, pp. 327.
  • MAJ, 2016. Wasted date palm. Ministry of Agriculture Jihad of Iran.
  • McDonald, P., Henderson, A.R., Heron, S.J.E., 1991. The Biochemistry of Silage. 2nd ed., Marlow, Bucks, UK. pp. 451-466.
  • Muck, R., 1988. Factors influencing silage quality and their implications for management. Journal of Dairy Science 71, 2992–3002.
  • Nagel, S.A., Broderick, G.A., 1992. Effect of formic acid or formaldehyde treatment of alfalfa silage on nutrient utilization by dairy cows. Journal of Dairy Science 75, 140–154.
  • Ogimoto, K., Imai, S., 1981. Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo, Japan.
  • Rajabi, R., Tahmasbi, R., Dayani, O., Khezri, A., 2016. Chemical composition of alfalfa silage with waste date and its feeding effect on ruminal fermentation characteristics and microbial protein synthesis in sheep. Journal of Animal Physiology and Animal Nutrition 101, 466-474.
  • Repetto, J.L., Cajarville, C., D’Alessandro, J., Curbelo, A., Soto, C., Garin, D., 2005. Effect of wilting and ensiling on ruminal degradability of temperate grass and legume mixtures. Animal Research 54, 73-80.
  • Reynolds, C.K., Kristensen, N.B., 2008. Nitrogen recycling through the gut and the nitrogen economy of ruminants: an asynchronous symbiosis. Journal of Animal Science 86, 293-305.
  • Rihani, N., Garrett, W.N., Zinn, R.A., 1993. Effect of source of supplemental nitrogen on the utilization of citrus pulp based diets by sheep. Journal of Animal Science 71, 2310-2321.
  • Russell, J.B., Wilson, D.B., 1996. Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH. Journal of Dairy Science 79, 1503–1509.
  • Russell, J.B., O’Conner, J.D., Fox, D.G., Van Soest, P.J., Sniffen, J., 1992. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science 70, 3551–3561.
  • Salisbury, M., Krehbiel, C., Ross, T., Schultz, C., Melton, L., 2004. Effects of supplemental protein type on intake, nitrogen balance, and site, and extent of digestion in whiteface withers consuming low-quality grass hay. Journal of Animal Science 82, 3567–3576.
  • SAS, 2005. SAS User’s Guide. SAS Institute Inc. Version 9. 1. Cary, NC, USA.
  • Salariniya, A., Fathinasari, M.H., Farhangfar, H., Naaeimipour Yonesi, H., 2012. Effect of different levels of dietary fiber starting on feed intake, daily gain, feed efficiency and rumen parameters of Holstein dairy calves. Iranian Journal of Animal Science Research 4, 323-334. (In Farsi)
  • Sidhu, J.S., 2012. Production and Processing of Date Fruits, Handbook of Fruits and Fruit Processing, 2nd ed. Wiley-Blackwell, New York, USA, pp. 629–651.
  • Sniffen, C.J., O’Connor, J.D., Van Soest, P.J., Fox, D.G., Russell, J.B., 1992. A net carbohydrate and protein system for evaluating cattle diets. II. Carbohydrate and protein availability. Journal of Animal Science 70, 3562–3577.
  • Touqir, N.A., Ajmal Khan, M., Sarwar, M., Nisa, M., Lee, W.S., Lee, H.J., Kim, H.S., 2007. Influence of varying dry matter and molasses levels on Berseem and Lucerne silage characteristics and their in situ digestion kinetics in Nili buffalo bulls. Asian-Australasian Journal of Animal Sciences 20, 887– 893.
  • Umana, R.C., Staples, R., Bates, D.B., Wilcox, C.J., Mahanna, W.C., 1991. Effects of a microbial inoculant and (or) sugar cane molasses on the fermentation, aerobic stability and digestibility of Bermudagrass ensiled at two moisture contents. Journal of Animal Science 69, 4588-4601.
  • Van Soest, P.J., 1994. Nutritional Ecology of the Ruminant. 2nd ed. Ithaca, NY, USA.
  • Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
  • Wang, L., Sun, Q.Z., Zhang, H.J., 2011. A study on quality of mixed silage of alfalfa and corn. Acta Prataca Sinica 20, 202-209.
  • Wen, A.Y., Yuan, X.J., Wang, J., Wang, J., Shao, T., 2011. Study on fermentation quality of mixed silage of alfalfa and Italian ryegrass. Journal of Anhui Science and Technology University 25, 10-14.
  • Xue, Z.L., Luo, F.C., Kuang, C.Y., Huang, B.Z., 2013. Analysis to mixed silage effect of Sorghum-Sudangrass hybrid and alfalfa. Journal of Yunn-Horng Agricultur University 28 340-345.
  • Zeng, L., Yan, J.Y., Zhang, X.F., Yang, X.Y., Zhao, Z., 2011. Research of mixed silage on alfalfa and Reed. Xin Animal Husbandry 1, 19-21.
  • Ziaei, N., 2010. The effect of dietary Alhagi (camel grass) ensiled with different levels of low quality date palm on apparent nutrient digestion coefficients in Kermani sheep. Research Journal of Biological Sciences 5, 314-317.