Seasonal variation in seminal characteristics, testicular measurements and plasma testosterone concentration in Iranian Khalkhali bucks

Document Type : Original Research Article (Regular Paper)

Authors

1 Department of Animal Science, College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Ministry of Jahad-e- Agriculture, Ardabil, Iran.

3 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.

4 Department of Animal Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran.

Abstract

The aim of the present study was to determine seasonal variations in semen characteristics, testicular size, and plasma testosterone concentration in Iranian Khalkhali goats. An artificial vagina was used to collect semen from 10 bucks (2–4 years of age), from November 2013 to October 2014. Testicular circumference was higher (P<0.05) from September to October. Ejaculate volume, sperm concentration, and percentage of normal sperm were higher (P<0.05) during September and October and lower during December and January (P<0.05). Plasma testosterone concentration was higher during October (P<0.05) than other months. Testicular circumference was positively correlated with serum testosterone concentration, percentage of live and normal sperm and semen volume. The lowest level of lactate dehydrogenase (LDH) in the seminal fluid (1.92 U per mL) was recorded during early October (autumn) and the highest level in early spring (2.80 U per mL). The lowest levels of K+ and Na+ in the seminal fluid were recorded in spring (59 and 72.3 mg per dL, respectively). The correlation coefficients of live and normal sperm percentage and sperm motility with seminal fluid K+ level (r = 0.60 and 0.46, respectively) and Na+ level (r = 0.48 and 0.35, respectively) were positive, and with LDH (r = -0.50 and -0.42, respectively) were negative (P<0.01). Despite monthly variations, semen quality was within the range regarded as satisfactory for normal fertility; however, the highest quality semen was collected during September and October.

Keywords

References

  • Ahmad, N., Noakes, D.E., 1995. Seasonal variations in testis size, libido and plasma testosterone concentrations in British goats. Journal of Animal Science 61, 553–559.
  • Al-Ghalban, A.M., Tabbaa, M.J., Kridli, R.T., 2004. Factors affecting semen characteristics and scrotal circumference in Damascus bucks. Small Ruminant Research 53, 141–149.
  • Ali, B.H., Mustafa, A.I., 1986. Semen characteristics of Nubian goats in Sudan. Animal Reproduction Science 12, 63-68.
  • Barkawi, A.H., Eitedal, H., Ashour, A., Shehata, E., 2006. Seasonal changes in semen characteristics, hormonal profiles and testicular activity in Zaraibi goats. Small Ruminant Research 66, 209-213.
  • Corteel, J.M., 1977. Production, storage and insemination of goat semen. In: Proceedings on Management of Reproduction in Sheep and Goats Symposium. University of Wisconsin, Madison, pp. 41-57. 
  • Delgadillo, J.A., Chemineau, P., 1992. Abolition of the seasonal release of luteinising hormone and testosterone in Alpine male goats (Capra hircus). Journal of Reproduction and Fertility 94, 45-55.
  • Duan, C., Goldberg, E., 2003. Inhibition of lactate dehydrogenase C4 (LDH-C4) blocks capacitation of mouse sperm in vitro. Cytogenetic and Genome Research 103, 352–359.
  • Dube, G.D., Dwaraknath, P.K., Vyas, K.K., 1982. Lactate dehydrogenase in relation to semen quality. Indian Journal of Dairy Science 35, 80–82.
  • Farshad, A., Yousefi, A., Moghaddam, A., Khalili, B., 2012. Seasonal changes in serum testosterone, LDH concentration and semen characteristics in Markhoz goats. Asian-Australian Journal of Animal Sciences 25,189 - 193
  • Hafez, E.S.E., Hafez, B., 2000. Reproductive cycles. In:. Hafez, E.S.E, Hafez, B. (Eds.) Reproduction in Farm Animals. 7th Ed. Lippincott, Williams and Wilkins, Philadelphia., pp. 55–67.
  • Hammoudi, S.M., Aït-Amrane, A., Belhamiti, T.B., Khiati, B., Niar, A.,Guetarni, D., 2010. Seasonal variations of sexual activity of local bucks in western Algeria. African Journal of Biotechnology 9, 3, 362-368.
  • Jafaroghli, M., Abdi-Benemar, H., Zamiri, M.J., Khalili, B., Farshad, A., Shadparvar, A.A., 2014. Effects of dietary n-3 fatty acids and vitamin C on semen characteristics, lipid composition of sperm and blood metabolites in fat-tailed Moghani rams. Animal Reproduction Science 147, 17–24.
  • Karagiannidis, A., Varsakeli, S., Karatzas, G., 2000. Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascus goat bucks born and raised in Greece.  Theriogenology 53, 1285-1293.
  • Langford, G.A., Sanford, L.M., Maqrcus, G.J., Shrestha, J.N.B., 1999. Seasonal cyclic pituitary and testicular activities in rams. Small Ruminant Research 33, 43-53.
  • Leboeuf, B., Delgadillo, J.A., Manfredi, E., Piacere. A., Clement, V., 4 , Martin, P., Pellicer, M., Boue, P., de Cremoux, R., 2008. Management of goat reproduction and insemination for genetic improvement in France. Reproduction in Domestic Animals 43, (Suppl. 2), 379–385.
  • Mendoza, G., White, I.G., Chow, P., 1989. Studies of chemical components of Angora goat seminal plasma. Theriogenology 32, 455-466.
  • Noran, A.M., Mukherejee, T.K., Abdullah, R., 1998. Semen characteristics of local Katjang and cross-bred (Katjang x German) bucks. Asian-Australian Journal of Animal Sciences 11, 445–449.
  •  Pandey, R.P., Sinha, S.N., Singh, B., Akhtar, M.H., 1985. Characters of semen and fertility rate in Saanen and Barbari bucks. Indian Journal of Animal Science 55, 773–774.
  • Roca, J., Martinez, E., Sanchez-Valverde, M.A., Ruiz, S., Vasquez, J.M., 1992. Seasonal variations of semen quality in male goats: study of sperm abnormalities. Theriogenology 38, 115-125.
  • SAS, 2003. SAS/STAT Software: Changes and Enhances Through Release 9.1.3. SAS Institute Inc., Cary, NC, USA.
  • Shamsuddin, M., Amiri, Y., Bhuiyan, M.M.U., 2000. Characteristics of buck semen with regard to ejaculate numbers, collection intervals, diluents and preservation periods. Reproduction in Domestic Animals 35, 53-57.
  • Stamatiadis, K., Karayanidis, A., Tsakalof, P., 1984. Relationship between LDH activity in seminal plasma and viability of ram spermatozoa. In; Courot, M. (Ed.),The Male in Farm Animal Reproduction. Martinus-Nijhoff Publishers, Boston, pp. 257–263.
  • Talebi, J., Souri, M., Moghaddam, A., Karimi, I., Mirmahmoodi, M., 2009. Characteristics and seasonal variation in the semen of Markhoz bucks in western Iran. Small Ruminant Research 85, 18–22.
  • Todini, L., Malfatti, A., Terzano, G.M., Borghese, A., Pizzillo, M., Debenedetti, A., 2007. Seasonality of plasma testosterone in male of four Mediterranean goat breeds and in three different climatic conditions. Theriogenology 67, 627–631.
  • Zamiri, M.J., Heidari, A., 2006.  Reproductive characteristics of Rayini male goats of Kerman province in Iran. Animal Reproduction Science 96, 176-185.
  • Zamiri, M.J., Khodaei, H.R., 2005. Seasonal thyroidal activity and reproductive characteristics of Iranian fat-tailed rams. Animal Reproduction Science 88, 245–255.
  • Zamiri, M.J., Khalili, B., Jafaroghli, M., Farshad, A., 2010. Seasonal variation in seminal parameters, testicular size, and plasma testosterone concentration in Iranian Moghani rams. Small Ruminant Research 94, 132–136.
Journal of Livestock Science and Technologies
Volume 6, Issue 2
December 2018
Pages 33-39

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