Effects of storage duration at 5 ºC and type of cryoprotectant on cooled and frozen-thawed ram epididymal spermatozoa

Document Type : Research Article (Regular Paper)

Authors

Department of Animal Science, College of Agriculture, University of Kurdistan, Sanandaj-Iran.

Abstract

Freezing of ejaculated and epididymal spermatozoa is currently a subject of interest with the purpose of establishing an efficient gene banking model for valuable animals or endangered species. Therefore, the present study evaluated the influence of different storage duration )0, 1.5 and 5 h) at 5 ºC and type of cryoprotectant on freezability of ram epididymal spermatozoa. With increasing the storage duration from 0 to 3 h at 5 ºC, the motility, progressive motility, viability and recovery rate of stored spermatozoa decreased (P<0.05), but acrosome integrity and hyaluronidase activity were not affected (P>0.05). Addition of trehalose and sucrose to the basic diluent improved (P<0.05) the quality of frozen-thawed spermatozoa. Sperm motility, progressive motility, viability and hyaluronidase activity were higher in the present of DMSO than the control and BSA. There was no difference in the acrosomal integrity between extenders. In conclusion, quality of froze-thawed spermatozoa was not improved when the extender was supplemented with sugar. However, further studies are needed to determine the fertility of frozen-thawed epididymal spermatozoa


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References

  • Abdelhakeam, A.A., Graham, E.F., Vazquez, A.I., Chaloner, K.M., 1991. Studies on the absence of glycerol in unfrozen and frozen ram semen: Development of an extender for freezing effect of osmotic pressure, egg yolk levels, type of sugars, and method of dilution. Cryobiology 28, 43-49.
  • Aboagla, E.M., Terada, T., 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biology of Reproduction 69, 1245-1250.
  • Aguado, M.J., Garde, J., Madriadano, J.M., Perez, S., Garrido, D., Montoro V., 1994. Congelacio´n post mortem de semen de epidıdimo de morueco. Proceedings of the 7th Jornadas Int Reprod Anim e IA, Murcia, Spain.
  • Aisen, E. G., Medina, V.H., Venturino, A., 2002. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 57, 1801-1808.
  • Alvareza, M., Tamayo-Canula, J., Martinez-Rodrigueza, C., Lopez-Uruena, E., Gomes-Alvesa, S., Anela, L., Martinez-Pastora, F., De Paza, P., 2012. Specificity of the extender used for freezing ram sperm depends of the spermatozoa source (ejaculate, electroejaculate or epididymis). Animal Reproduction Science 132, 145–154.
  • Amann, R.P., Hay, S.R., Hammersted, R.H., 1982. Yield characteristics, motility and cAMP content of sperm isolated from seven regions of ram epididymis. Biology of Reproduction 27, 723-733.
  • Bucak, M.N., Necmettin, T., 2007. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research 73, 103-108.
  • Chen, Y., Foote, R.H., Brockett, C.C., 1993. Effect of sucrose, trehalose, hypotaurine, taurine and blood serum on survival of frozen bull sperm. Cryobiology 30, 423- ­431.
  • Drobnis, E. Z., L. M. Crowe, T. Berger, T. J. Anchordoguy, J. W. Overstreert and J. H. Crowe. 1993. Cold shock damage is due to lipid phase-transitions in cell membranes demonstration using sperm as a model. Journal of Experimental Zoology 265, 432-437.
  • Evans, G., Maxwell, W.M.C., 1987. Salamon’s Artificial Insemination of Sheep and Goats. University Sydney, NSW, Australia.
  • Farshad, A., Akhondzadeh, A., 2008. Effects of sucrose and glycerol during the freezing step of cryopreservation on the viability of goat spermatozoa. Asian-Australasian Journal of Animal Sciences 21, 1721-1727.
  • Foote, R.H., 2000. Letter to the Editor: Fertilizing ability of epididymal sperm from dead animals. Journal of Andrology 21, 355.
  • Foote, R.H., Chen, Y., Brockett, C.C., 1993. Fertility of bull spermatozoa frozen in whole milk extender with trehalose, taurine or blood serum. Journal of Dairy Science 76, 1908-1913.
  • Foulkes, J.A., Watson, P.A. 1995. Hyaluronidase activity in seminal plasma as a method of assessing bull sperm integrity. Journal of Reproduction and Infertility 43, 349-353.
  • Hishinuma, M., Suzuki, K., Sekine, J., 2003. Recovery and cryopreservation of sika deer (Cervus nippon) spermatozoa from epididymides stored at 4ºC. Theriogenology 59, 813-820.
  • Johnson, L., Amann, R.P., Pickett, B.W., 1980. Maturation of equine epididymal spermatozoa. American Journal of Veterinary Research 41, 1190–1196.
  • Kaabi, M., Paz, P., Alvarez, M., Anel. E., 2003. Effect of epididymis handling conditions on the quality of ram spermatozoa recovered post-mortem. Theriogenology 60, 1249-1259.
  • Karja, N.W.K., Respat, E.M.A., Nuraini, I., Prihatno, S.A., Gustari, S., 2010. Characteristic of frozen-thawed epididymal spermatozoa and refrigerated storage of ram spermatozoa. Journal of the Indonesian Tropical Animal Agriculture 35, 63-67.
  • Khalili, B., Farshad, A., Zamiri, M.J., Rashidi, A., Fazeli, P., 2009. Effects of sucrose and trehalose on the freezability of Markhoz goat spermatozoa. Asian-Australasian Journal of Animal Sciences 22, 1614–1619.
  • Khalili, B., Jafaroghli, M., Farshad, A., Paresh-Khiavi, M., 2010. The effects of different concentrations of glycine and cysteine on the freezability of Moghani ram spermatozoa. Asian-Australasian Journal of Animal Sciences 23, 318–325.
  • Kikuchi, K., Nagai, T., Kashiwazaki, N., Ikeda, H., Noguch,i J., Shimada, A., 1998. Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4ºC. Theriogenology 50, 615–23.
  • Kishikawa, H., Tateno, H., Yanagimachi, R., 1999. Fertility of mouse spermatozoa retrieved from cadavers and maintained at 4 ºC. Journal of Reproduction and Fertility 116, 217–22.
  • Liu, Z., Foote, R.H., Brockett, C.C., 1998. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology 37, 219-230.
  • McGinnis, L.K., Zhu, L., Lawitts, J.A., Bhowmick, S., Tone,r M., Biggers, J.D.,  2005. Mouse sperm -desiccated and stored in trehalose medium without freezing. Biology of Reproduction 73, 627-633.
  • Molinia, F.C., Maxwell, W.M.C., 1994. Effect of monosaccharides and disaccharides in tris based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa. Animal Reproduction Science 36, 113-22.
  • Monteiro, G.A., Papa, F.O., Zahn, F.S., Dellaqua, J.A., Melo, C.M., Maziero, R.R.D., Avanzi, B.R., Alvarenga, M.A., Guasti, P.N., 2011. Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Animal Reproduction Science 127, 197-201.
  • Nagai, T., Takahashi, T., Masuda, H., Shioya, Y., Kuwayama, M., Fukushima, M., Iwasaki, S., Hanada, A., 1988. In-vitro fertilization of pig oocytes by frozen boar spermatozoa. Journal of Reproduction and Fertility 84, 585-591.
  • Nakagata, N., 1992. Production of normal young following insemination of frozen-thawed mouse spermatozoa into fallopian tubes of pseudopregnant female. Experimental Animals 41, 519-522.
  • Parrish, J.J., Susho-parrish, J., Winer, M.A., First, N.L., (1988). Capacitation of bovine sperm by heparin. Biology of Reproduction 38, 1171-1180.
  • Purdy, P.H., 2006. A review on goat sperm cryopreservation. Small Ruminant Research 63, 215-225.
  • Purdy, P.H., Fox M.H., Graham J.K., 2005. The fluidity of Chinese hamster ovary cell and bull sperm membranes after cholesterol addition. Cryobiology 51, 102-112.
  • O’Hara, L., Hanrahan, J.P., Richardson, L., Donovan, A., Fair, S., Evens, A.C.O., Lonergan, P., 2010. The effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm. Theriogenology 73, 541-549.
  • Salamon, S., Maxwell, W.M., 2000. Storage of ram semen. Animal Reproduction Sciences 62, 77-111.
  • SAS. 1996. SAS/STAT Software: Changes and enhancements through release 6. 12. SAS Institute Inc., Cary, NC, USA.
  • Varisli, O., Uguz, C., Agca, C., Agca, Y., 2009. Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Animal Reproduction Science 110, 256-268.
  • Vicente, J.S., Viudes-de-Castro, M.P., 1996. A sucrose-DMSO extender for freezing rabbit semen. Reproduction, Nutrition and Development 36, 485-492.
  • Watson, P.F., 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thaw function. Reproduction Fertility and Development 7, 871-891.
  • Watson, P.F., 2000. The causes of reduced fertility with cryopreserved semen. Animal Reproduction Sciences 60, 481-492.
  • Woelders, H., Matthijs, A., Engel, B., 1997. Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35, 93-105.
  • Yildiz, C., Kaya, A., Aksoy, M., Tekeli, T., 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54, 579-585.
Journal of Livestock Science and Technologies
Volume 5, Issue 2
December 2017
Pages 29-34

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