Shahid Bahonar University of Kerman and Iranian Society of Animal Science Journal of Livestock Science and Technologies 2322-3553 14 2 2026 02 01 Polymorphism of pyruvate carboxylase gene and its relation with productive and reproductive performance of dairy cows 17 23 4956 10.22103/jlst.2025.25042.1616 EN Waleed Mohammed Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq Haitham Hussein Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq Tahreer Al-Thuwaini Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq Journal Article 2025 04 01 Genetic selection in dairy cattle can be enhanced by identifying the candidate genes and variants associated with economic traits. This study examined the productive and reproductive characteristics of Holstein cattle based on the genotypes of the pyruvate carboxylase (<em>PC</em>) gene. A total of 50 cows, aged three to five years, was included in the study, with identical feeding and maintenance protocols applied to all animals. All cows completed a lactation period of 305 days. The genotype and allele frequencies were determined through genotyping. Sequencing analysis of 816 bp amplicons detected two single-nucleotide polymorphisms (SNPs), rs42195003 and rs110476776. The association analysis of the two SNPs was not significantly associated with lactation period, dry period, milk composition<strong>, </strong>and reproductive performance (<em>P</em>>0.05). In contrast, the highest milk production (4305.63±222.01 kg), was associated with the CT genotype of the <em>PC</em> (rs110476776) polymorphism. The results revealed that the SNP rs110476776 in the <em>PC</em> gene was associated with total milk production in Holstein cows. This study may provide valuable insights into the role of the analyzed gene in the functional traits of dairy cattle; however, it is essential to validate the results by researching larger populations and different breeds of cattle. https://lst.uk.ac.ir/article_4956_ba00f6fd1171d731d1f8301a56df7a1e.pdf